A novel antimicrobial-containing nanocellulose scaffold for regenerative endodontics
Kichler Victoria, Teixeira Lucas Soares, Prado Maick Meneguzzo, Colla Guilherme, Schuldt Daniela Peressoni Vieira, Coelho Beatriz Serrato, Porto Luismar Marques, de Almeida Josiane,
소속 상세정보
( Kichler Victoria ) - University of Southern Santa Catarina Faculty of Dentistry Department of Endodontics
( Teixeira Lucas Soares ) - University of Southern Santa Catarina Faculty of Dentistry Department of Endodontics
( Prado Maick Meneguzzo ) - Federal University of Santa Catarina Department of Chemical Engineering
( Colla Guilherme ) - Federal University of Santa Catarina Department of Chemical Engineering
( Schuldt Daniela Peressoni Vieira ) - University of Southern Santa Catarina Faculty of Dentistry Department of Endodontics
( Coelho Beatriz Serrato ) - University of Southern Santa Catarina Faculty of Dentistry Department of Endodontics
( Porto Luismar Marques ) - Federal University of Santa Catarina Department of Chemical Engineering
( de Almeida Josiane ) - University of Southern Santa Catarina Faculty of Dentistry Department of Endodontics
Abstract
Objectives: The aim of this study was to evaluate bacterial nanocellulose (BNC) membranes incorporated with antimicrobial agents regarding cytotoxicity in fibroblasts of the periodontal ligament (PDLF), antimicrobial activity, and inhibition of multispecies biofilm formation.
Materials and Methods: The tested BNC membranes were BNC + 1% clindamycin (BNC/CLI); BNC + 0.12% chlorhexidine (BNC/CHX); BNC + nitric oxide (BNC/NO); and conventional BNC (BNC; control). After PDLF culture, the BNC membranes were positioned in the wells and maintained for 24 hours. Cell viability was then evaluated using the MTS calorimetric test. Antimicrobial activity against Enterococcus faecalis, Actinomyces naeslundii, and Streptococcus sanguinis (S. sanguinis) was evaluated using the agar diffusion test. To assess the antibiofilm activity, BNC membranes were exposed for 24 hours to the mixed culture. After sonicating the BNC membranes to remove the remaining biofilm and plating the suspension on agar, the number of colony-forming units (CFU)/mL was determined. Data were analyzed by 1-way analysis of variance and the Tukey, Kruskal-Wallis, and Dunn tests (α = 5%).
Results: PDLF metabolic activity after contact with BNC/CHX, BNC/CLI, and BNC/NO was 35%, 61% and 97%, respectively, compared to BNC. BNC/NO showed biocompatibility similar to that of BNC (p = 0.78). BNC/CLI showed the largest inhibition halos, and was superior to the other BNC membranes against S. sanguinis (p < 0.05). The experimental BNC membranes inhibited biofilm formation, with about a 3-fold log CFU reduction compared to BNC (p < 0.05).
Conclusions: BNC/NO showed excellent biocompatibility and inhibited multispecies biofilm formation, similarly to BNC/CLI and BNC/CHX.
키워드
Anti-infective agents; Biofilms; Regenerative endodontics; Tissue scaffolds
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